Yeast Co-Culture with Trichoderma harzanium ATCC® 20846™ in Submerged Fermentation Enhances Cellulase Production from a Novel Mixture of Surgical Waste Cotton and Waste Card Board

Abstract

Trichoderma harzanium ATCC® 20846™ when used in the process of submerged fermentation for the production of cellulases, suffers a catabolite repression due to the accumulation of glucose within the fermentation broth. A strain of yeast Saccharomyces cereviseae RW 143 was inoculated into the cellulase production fermentation containing T. harzanium ATCC® 20846™. In two different experiments yeast (5% w/v) whole cells and immobilized yeast cells (5%w/v) were used. The accumulated glucose was consumed by the yeast and T. harzanium ATCC® 20846™ was efficiently able to produce enzymes with increased activities. When no yeast cells were added, 1.946 FPU/mL was obtained at the end of 168 hr. When yeast whole cells were used 2.035 FPU/mL was obtained at the end of 96 hr and when calcium alginate-immobilized yeast cells were used 1.75 FPU/mL was obtained at the end of 120 hr. The 0.09 g/L ethanol was produced at the end of 96 hr when whole yeast cells were used for co-culture.

Keywords

Trichoderma harzanium; Cellulase production; Saccharomyces cereviseae; Co-culture

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